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    OptiPharma Protein Solubility Screening Kit

    $269.00$749.00

    Name Details
    OptiPharma l Peptides, size   < 9 kDa
    OptiPharma ll Small protein molecules   > 10 kDa and < 25 kDa
    OptiPharma lll Protein molecules upto 90kDa
    OptiPharma lV Large protein molecules including antibodies and oligomers up to 250 kDa

    Select the Filter Plate according to the expected molecular weight of the protein molecule.

    Additional Discount: Order 12 or more Kits, an additional 5% discount will be applied at checkout.


    Discount Per Quantity

    Quantity 12 +
    Price $255.55$711.55
    Clear
    Catalog: OptiPharma

    DESCRIPTION

    OptiPharma™ Protein Solubility Kits help researchers to identify formulations that protect target biopharmaceutical from aggregation, or to solubilize an aggregated biopharmaceutical sample.

    OptiPharma Protein Solubility Screening Kit contains a combination of 10 types of buffers (pH varies from 3 to 8.5) and 13 pharmaceutical excipients (salts, amino acids, sugars, surfactants, preservatives). A total of 93 different formulations in a 96-well format can be tested in single, label-free experimental setup. The remaining 3 wells are for control experiments. The table below lists the formulations in the OptiPharma Kit.


    Click here to access the OptiPharma Quickstart Guide!
    Click here to access the OptiPharma Manual!

    Why OptiPharma?

    OptiPharma™ Protein Solubility Kits help researchers in various steps of biopharmaceutical development to identify formulations that protect precious biopharmaceuticals from aggregation, or to solubilize an aggregated biopharmaceutical sample.
    • Quickly evaluate aggregation behavior as a function of buffer types, pH, and excipients
    • Assay more than 90 different conditions in parallel and label-free
    • Simple mix and spin protocol, no ultracentrifugation required
    • Opportunity to rescue reversibly aggregated samples
    • Protect your biopharmaceutical from aggregation under stress*
    • Quickly find trends with the Pharma Dashboard
    Proteins aggregate, This undesired process is often caused by elevated temperature, vigorous stirring, addition of ligands or molecular binding partners or just by mere storage of a protein sample over a period of time. Protein aggregation can often be avoided with proper choice of pH, salt or stabilizing additives. OptiPharma Protein Solubility Screening Kit contains a combination of 10 types of buffers (pH varies from 3 to 8.5) and 13 pharmaceutical excipients (salts, amino acids, sugars, surfactants, preservatives). A total of 93 different formulations in a 96-well format can be tested in single, label-free experimental setup. The remaining 3 wells are for control experiments. Soluble proteins pass filters, aggregated proteins do not. This simple feature is exploited to in the filtration step. The wells that contain soluble proteins will yield protein after the filtration process; those with protein aggregates will not contain any protein. After running the OptiPharma Kit you have established conditions that keep your biopharmaceutical target in solution and you know conditions at which your protein aggregates.
    • Better and faster than a conventional trial-and-error testing
    • Low protein consumption consumes about 10 µL for each protein solubility data point. However, the protein quantity should be matched with your method of detection.
    • Convenient: Obtain a lot of information on protein solubilization in one experiment. Utilize the target specific assay of your own choice. The OptiPharma Protein Solubility kit is compatible with many functional assays, SDS-PAGE, Western blot, mass spectrometry, and fluorescence-based protein detection.

    Applications

    Applications for the OptiPharma™ Protein Solubility Screening Kit
    The OptiPharma™ Protein Solubility Screening Kit can be used to protect target proteins against a variety of aggregation-inducing stresses. Proteins often aggregate when exposed to elevated temperature, when they are stored for longer periods of time, when protein solutions are repeatedly frozen and thawed or when the liquid is exposed to high shear forces or intense light. Oxidation by air, reactive reagents, and surface exposure are also causes of protein aggregation. These stressors can be used to assess the susceptibility of a particular protein sample and identify protective agents. OptiPharma™ Protein Solubility Screening Kit can provide you answers, If your challenge is –
    • Undesired antibody or protein aggregation
    • Reversible precipitation of proteins during elution off a purification column
    • Insoluble protein at neutral pH
    If you need to –
    • Screen different buffers to maintain your protein stable at high concentrations
    • Keep an antigen soluble while removing certain components from the buffer
    • Separate bound antigen-antibody complex into unbound forms
    • Find optimal conditions for long term stability of proteins following purification.
    • Optimize purification conditions

    Technology

    THIS IS HOW THE OPTIPHARMA KITS WORK:

    HOW THE OPTIPHARMA KITS WORK
    NEW FASOS TECHNOLOGY

    OptiPharma, similar to OptiSol, is based on proprietary FASOS Technology. FASOS stands for Filter Aided Solubility Optimization Screening. A protein in original buffer condition is mixed separately with various OptiPharma formulations. Protein in formulations that promote solubilization will be detected in the filtrates after passing through a specific molecular weight cut-off filter. On the other hand, aggregated protein due to ‘bad’ formulations is retained on the filter. This combinatorial solution and filtration approach identifies the optimal pH, buffer type, and additives that maintain samples solubilized. One can either solubilize an aggregated protein solution or select a particular sample stress (heat, freeze-thaw, storage, shear etc.) and identify those solutions that keep the protein sample solubilized without aggregation. Data evaluation is supported with Pharma Dashboard.


    Application A: Protein Solubility Profiling
    Protein-Solubility-Profiling
    Application B: Protein Aggregate Solubilization
    Protein-AggregateSolubilization
    HOW MUCH PROTEIN IS REQUIRED?

    The protein concentration will be diluted by 11 times (15 µL/165 µL) in the reaction plate when the protein solution is combined with the formulations. Thus, one has to start with a stock protein solution that is 11 times the final solution concentration in the reaction plate. About 2 mL of protein stock solution is adequate. The required protein concentration depends on the extinction coefficient of the protein and the sensitivity of the protein detection assay. In our laboratory we developed a UV280 method using half-width UV 96-well plate and UV/vis plate reader. In our case, the UV280 absorbance in half-width UV280 wells has to be at least around 0.4 (absolute absorbance units).
    Click here to access the OptiPharma Manual!

    Which OptiPharma™ ?

    The OptiPharma™ Protein Solubility Screening kit is available for four different sizes of proteins as described below. Each of these kits is also available in pack of 3 (3X).  
    Catalog Product Name Protein Size Price
    SOL-Pharm-l OptiPharma Protein Solubility Screening Kit I Peptides, size  < 9 kDa $269
    SOL-Pharm-l-3X OptiPharma Protein Solubility Screening Kit I (3X) Peptides, size  < 9 kDa $749
    SOL-Pharm-ll OptiPharma Protein Solubility Screening Kit II Small protein molecules   > 10 kDa and < 25 kDa $269
    SOL-Pharm-ll-3X OptiPharma Protein Solubility Screening Kit II (3X) Small protein molecules   > 10 kDa and < 25 kDa $749
    SOL-Pharm-lll OptiPharma Protein Solubility Screening Kit III Protein molecules upto 90kDa $269
    SOL-Pharm-lll-3X OptiPharma Protein Solubility Screening Kit III (3X) Protein molecules upto 90kDa $749
    SOL-Pharm-lV OptiPharma Protein Solubility Screening Kit IV Large protein molecules including antibodies and oligomers up to 250 kDa $269
    SOL-Pharm-lV-3X OptiPharma Protein Solubility Screening Kit IV (3X) Large protein molecules including antibodies and oligomers up to 250 kDa $749
     

    Data Analysis

    Easy Data Analysis with the Pharma Dashboard
    Pharma Dashboard™ is supplied with the OptiPharma Protein Solubility Screening Kit. This tool aids in spotting the trends of protein behavior and provides identification of critical solvent factors for optimal protein solubilization within a single experiment. Just cut-and-paste your output data into the Excel-based Pharma Dashboard and see your target protein behavior. Contact us for more information on how to access the link for the Pharma Dashboard. info@solublebioscience.com

    FAQ

    OptiPharma vs. OptiSol: Which one I should get?
    All ingredients (buffers and additives) in an OptiPharma kit (except 3 control wells) are FDA approved pharmaceutical ingredients. If you have pharmaceutical proteins in any stages of pharmaceutical formulation development, OptiPharma is the one.
    OptiSol is applicable to various types of protein work (e.g. pharmaceutical, industrial, food, plant, crystallization work). Most additives in an OptiSol kit are not suitable for pharmaceutical formulations. For example, if you have a crude protein mixture that you want to solubilize, OptiSol is a good choice to start with.
    Will my aggregated protein sample ‘go in solution’ with the OptiPharma formulations?
    This depends on the nature of the protein aggregate. The only way to find out is by running an experiment, and this is exactly what the OptiPharma protein solubility screen does. OptiPharma Solubility Screening Kit tests 93 different formulations (different pH, salts, sugars, surfactants, and preservatives). If the protein sample is reversibly aggregated, chances are the OptiPharma screen will yield conditions that solubilize the sample.
    The goal is to solubilize the aggregate and yield functional protein. Will any aggregated protein samples get solubilized? No, some aggregates just contain ‘dead’ protein. You will need SDS or urea for such recalcitrant samples, and obtain non-functional protein in return.
    How much protein is required to run an OptiPharma kit?
    The protein concentration will be diluted by 11 times (15 µL/165 µL) in the reaction plate when the protein solution is combined with the formulations. Thus, one has to start with a stock protein solution that is 11 times the final solution concentration in the reaction plate. About 2 mL of protein stock solution is adequate. The required protein concentration depends on the extinction coefficient of the protein and the sensitivity of the protein detection assay. In our laboratory we developed a UV280 method using half-width UV 96-well plate and UV/vis plate reader. In our case, the UV280 absorbance in half-width UV280 wells has to be at least around 0.4 (absolute absorbance units).
    How do I analyze the results of the OptiPharma experiments?
    We advise to select a sensitive protein detection assay to characterize the filtrate, preferentially in 96-well format. In most cases plate reader data can be directly copy-and-pasted into the Pharma Dashboard™ Excel Spreadsheet to evaluate the results of the experiment and obtain graphical support of protein behavior trends and identify the critical solvent factors for optimal protein solubilization. Various analytical assays may be applied to determine the quantity or activity of protein in the filtered solution. The various assays may be based on detection of fluorescence or radioactivity (protein assay, enzyme assay, western/ELISA, ligand binding assay). Note that assays may require compensation for OptiPharma formulations or may not work in the presence of the OptiPharma formulations. Please consult the appropriate assay manual.
    Which Protein Assay works well with OptiPharma?
    Any protein assay that has sufficient specificity, dynamic range, and sensitivity may work with OptiPharma. Some researchers run dot-blots (Western), some have a fluorescence readout, employ an ELISA, or use an enzymatic activity assay. Our laboratory has developed a UV280 protocol for analyzing the filtrates from OptiPharma kit. This is up to the user and depends on what a user wants to screen for.
    Functional assays (measuring binding or enzymatic activity) or passive assays (measuring protein mass) can both be employed.
    Several users who have subjected purified protein samples have employed the Pierce BCA assay – and the ‘MICRO’ variant thereof.
    How long does the experiment take?
    It depends somewhat on the setup, but plan for approximately 3 hours to run the experiment.
    How many samples can I process with an OptiPharma kit /How many “runs” are in each kit?
    The OptiPharma protein solubility screening kit is designed to be run with a single protein sample only. While we have heard from users that have employed the filter plate several times successfully, we do not recommend it. Multiple uses will eventually clog the filter and resulted in unreliable results.
    How does the Pharma Dashboard help in analyzing my data?
    You are welcome to use any tool you want to graph and visualize your OptiPharma results. The Pharma Dashboard provides a quick overview and a detailed understanding of protein sample behavior
    What is the composition of the OptiPharma formulation plate?
    The OptiPharma formulation plate consists of a combinatorial solution set with a variety of pH, buffer types, and pharmaceutical additives. All chemicals are proven pharmaceutical ingredients, except the ones in control wells. A detailed list of all the components is also included in the OptiPharma Quickstart Guide (comes with the kit) and the Pharma Dashboard.