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    OptiSol Protein Solubility Screening Kit

    $269.00$749.00

    Name Details
    OptiSol I Peptides, size   < 9 kDa
    OptiSol II Small protein molecules   > 10 kDa and < 25 kDa
    OptiSol III Protein molecules upto 90kDa
    OptiSol IV Large protein molecules including antibodies and oligomers up to 250 kDa

    Select the Filter Plate according to the expected molecular weight of the protein molecule.

    Additional Discount: Order 12 or more Kits, an additional 5% discount will be applied at checkout.


    Discount Per Quantity

    Quantity 12 +
    Price $255.55$711.55
    Clear
    Catalog: OptiSol

    DESCRIPTION

    OptiSol™ Protein Solubility Kits help researchers identify formulations that protect target protein from aggregation, or to solubilize an aggregated protein sample.

    Why OptiSol ?

    Find your protein’s solubility sweet spot

    OptiSol™ Protein Solubility Kits help researchers identify formulations that protect target protein from aggregation, or to solubilize an aggregated protein sample.

    • Quickly evaluate aggregation behavior as a function of pH, salt and additives
    • Assay more than 90 different conditions in parallel and label-free
    • Simple mix and spin protocol, no ultracentrifugation required
    • Opportunity to rescue reversibly aggregated protein samples
    • Protect your protein from aggregation under stress*
    • Quickly find trends with the Protein Dashboard

    Proteins aggregate, This undesired process is often caused by elevated temperature, vigorous stirring, addition of ligands or molecular binding partners or just by mere storage of a protein sample over a period of time. Protein aggregation can often be avoided with proper choice of pH, salt or stabilizing additives. This OptiSol Protein Solubilization Screening Kit contains a systematically varied array of buffers (from pH 3 to pH 10) and a series of solubility enhancers (salts, amino acids, sugars, polyols, reducing reagents) that enable the determination of conditions under which a particular protein sample is protected from aggregation or can be de-aggregated. A total of 90 different formulations with solubility enhancers can be tested in one single, label-free experiment; the remaining 6 experiments are positive and negative control experiments.

    Soluble proteins pass filters, aggregated proteins don’t. This simple feature is exploited to in the filtration step. The wells that contain soluble proteins will yield protein after the filtration process; those with protein aggregates will not contain any protein.

    After running the Protein Solubilization Kit you have established conditions that keep your protein target in solution and you know conditions at which your protein aggregates. Then choose conditions to obtain a “well behaved protein”.

    • Better and faster than a conventional trial-and-error testing (less than 1 h)
    • Low protein consumption consumes less than 10 uL for each protein solubility data point. The protein quantity needs to be matched with your target detection assay
    • Convenient: get a lot of information on protein solubilization in one go. Utilize the target specific assay of your own choice. The OptiSol Protein Solubility Screening kit is compatible with many functional assays, SDS-PAGE, Western blot, mass spectrometry and fluorescence-based protein detection.

    Applications

    Applications for the Protein Solubility Screening Kit OptiSol™

    The OptiSol™ Protein Solubility Screening Kit can be used to protect target proteins against a variety of aggregation-inducing stresses. Proteins often aggregate when exposed to elevated temperature, when they are stored for longer periods of time, when protein solutions are repeatedly frozen and thawed or when the liquid is exposed to high shear forces or intense light. Oxidation by air, reactive reagents or surface exposure provide further sources of protein aggregation.

    These stressors can be used to assess the susceptibility of a particular protein sample and identify protective agents.

    If your challenge is –

    • Undesired antibody or protein aggregation
    • Reversible precipitation of proteins during elution off a purification column
    • Insoluble protein at neutral pH

    If you need to –

    • Screen different buffers to maintain your protein stable at high concentrations
    • Keep an antigen soluble while removing certain components from the buffer
    • Separate bound antigen-antibody complex into unbound forms
    • Find optimal conditions for long term stability of proteins following purification.
    • Optimize purification conditions

    Technology

    THIS IS HOW THE OPTISOL KITS WORK:
    how-optisol- works-image-1

    how-optisol-works-image-1

    NEW FASOS TECHNOLOGY

    OptiSol is based on proprietary FASOS Technology. FASOS stands for Filter Aided SolubilityOptimization Screening. A proteins solution is mixed separately with many different solutionsand only those protein solutions that pass a Molecular Weight Cutoff Filter (MWCO 30 or MWCO 200) are detected. This combinatorial solution and filtration approach identifies the optimal pH, salt, buffer type, additives and antioxidants that maintain samples solubilized. Either solubilize an aggregated protein solution (Application A) or select a particular sample stress (heat, freeze-thaw, storage, shear etc.) and identify those solutions that keep your protein sample solubilized without aggregation. Data evaluation is supported with the Protein Dashboard.

    Application A: Protein Solubility Profiling
    Application B: Protein Aggregate Solubilization

    how-optisol-works-application-a

    how-optisol-works-application-a

    how-optisol-works-application-b

    how-optisol-works-application-b

    Data Analysis

    Easy Data Analysis with the Protein Dashboard

    Together with the OptiSol Protein Solubility Screening Kit the Protein Dashboard™ is supplied. This new tool aids in spotting protein behavior trends and identify the critical solvent factors for optimal protein solubilization.

    Watch video now

    The Protein Dashboard™ aids in spotting protein behavior trends and identify the critical solvent factors for optimal protein solubilization within a single experiment. Just cut-and-paste your output data into the Excel-based Protein Dashboard and see your target protein behavior.

    Contact us for more information on information on how to access the link for the Protein Dashboard. info@solublebioscience.com

    Which OptiSol™ ?

    The OptiSol™ Protein Solubility Screening kit is available for four different sizes of proteins as described below. Each of these kits is also available in triplicate set (3X).

     

    Catalog Product Name Protein Size Price
    SOL-l OptiSol Protein Solubility Screening Kit I Peptides, size  < 9 kDa $269
    SOL-l-3X OptiSol Protein Solubility Screening Kit I (3X) Peptides, size  < 9 kDa $749
    SOL-ll OptiSol Protein Solubility Screening Kit II Small protein molecules   > 10 kDa and < 25 kDa $269
    SOL-lll-3X OptiSol Protein Solubility Screening Kit II (3X) Small protein molecules   > 10 kDa and < 25 kDa $749
    SOL-lll OptiSol Protein Solubility Screening Kit III Protein molecules upto 90kDa $269
    SOL-lll-3X OptiSol Protein Solubility Screening Kit III (3X) Protein molecules upto 90kDa $749
    SOL-lV OptiSol Protein Solubility Screening Kit IV Large protein molecules including antibodies and oligomers up to 250 kDa $269
    SOL-lV-3X OptiSol Protein Solubility Screening Kit IV (3X) Large protein molecules including antibodies and oligomers up to 250 kDa $749

     

    FAQ

    Will my aggregated protein sample ‘go in solution’ with the OptiSol reagents?

    This depends on the nature of the protein aggregate. The only way to find out is by experimental testing – and this is exactly what the OptiSol protein solubility screen does. Test 90 different mild formulations – different pH, salts, polymers, sugars, polyols, detergent, reducing agents. If the protein sample is reversibly aggregated, chances are the OptiSol screen will yield conditions that solubilize the sample.

    The formulations employed are rather mild. The goal is to solubilize the aggregate and yield functional protein. Will any aggregated protein samples get solubilized? No, some aggregates just contain ‘dead’ protein. You’ll need SDS or urea for such recalcitrant samples, and obtain non-functional protein in return.

    How do I analyze the results of the OptiSol experiments?

    We advise to select a sensitive protein detection assay to characterize the filtrate, preferentially in 96-well format. In most cases plate reader data can be directly copy-and-pasted into the Protein Dashboard™ Excel Spreadsheet to evaluate the results of the experiment and obtain graphical support of protein behavior trends and identify the critical solvent factors for optimal protein solubilization. Examples for diagnostic analytical tests that may be applied to determine the quantity or activity of protein in the filtered solution. The various assays may be based on detection of Fluorescence or Radioactivity (Protein Assay, enzyme assay, western/ELISA, ligand binding assay). Note that assays may require compensation for OptiSol formulations or may not work in the presence of the OptiSol formulations. Please consult the appropriate assay manual or utilize OptiSol formulations as controls.

    Which Protein Assay works well with OptiSol?

    Any protein assay that has sufficient specificity, dynamic range and sensitivity. Some researchers run dot-blots (Western), some have a fluorescence readout, employ an ELISA or use an enzymatic activity assay. This is up to you and depends on what you want to screen for.

    Functional assays (measuring binding or enzymatic activity) or passive assays (measuring protein mass) can both be employed.

    Several users who have subjected purified protein samples have employed the Pierce BCA assay – and the ‘MICRO’ variant thereof.

    How long does the experiment take?

    It depends somewhat on your setup, but plan for approximately 3 hours to run the experiments.

    How many samples can I process with an OptiSol kit /How many “runs” are in each kit?

    The OptiSol protein solubility screening kit is designed to be run with a single protein sample only. While we have heard from users that have employed the filter plate several times successfully, we don’t recommend it. Multiple use will eventually clog the filter and make the results unreliable.

    How does the protein dashboard help in analyzing my data?

    You’re welcome to use any tool you want to graph and visualize your OptiSol results. The Protein Dashboard is a quick way to plot dependencies of pH vs other parameters. This helps to get a quick overview and a detailed understanding of protein sample behavior.

    Check out Protein Dashboard video under Data Analysis Tab.

    What is the composition of the OptiSol reagents?

    The OptiSol reagents consist of a combinatorial solution set with a wide variety of pH, buffer types, charged & neutral organic additives and antioxidants. A detailed list of all the components are listed in the OpitSol brochure and the Protein Dashboard.

    What is Solubilisin II?

    Solubilisin II is a proprietary solution that has been shown to promote the solubility and protect proteins from aggregation over a wide range of pH values.

    Can Solubilisin II be used in PK studies in vitro?

    Solubilisin II has not been tested for in vivo studies and should be used for in vitro studies only. Some of the reagents supplied with the OptiSol kits contain low amounts of sodium azide and are hence incompatible with live cells. The OptiSol kit is only for in vitro use, not for cell-based or animal research.