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    8 Top Tips for Improving In Vitro Translation

    If you need a eukaryotic protein for functional studies, it makes sense to overexpress it in a cell-free system, which allows conditions closer to native including post-translational modifications. However, expressing protein in vitro does not exclude two major problems - low yield and sometimes even protein aggregation. Here are 8 tips to help you overcoming these problems. Figure 1. Flowchart ...
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    What Insects Did For Glycoprotein Purification

    A large number of biotechnologically relevant proteins, including enzymes, hormones, cytokines, clotting factors, monoclonal antibodies are biologically active only in the glycoprotein form (1). Glycoproteins - proteins with covalently attached oligosaccharide chains - are not the easiest type of proteins to work with. The oligosaccharide (glycan) part, important for the correct folding and/or biological activity of the purified protein, is ...
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    Three factors for choosing the correct pH for your protein purification buffer to prevent protein aggregation

    Before you purify your first batch of a new protein it is important to know what buffer you might want to use to keep precipitation of proteins to a minimum. Many cellular proteins are in a physiologic environment with neutral pH and relatively low salt concentrations, but very high concentration of other proteins. So you might think that a good ...
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    When My Protein Aggregates During Dialysis, This Is What Scientists On Linkedin Do

    What do scientists and their colleagues on LinkedIn do when their protein aggregates during dialysis? LinkedIn poll with results as of 7 May 2013. Submit your own vote in the live poll to see updated results ...
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    Help: My freaky protein doesn’t have structure. And it aggregates!

    Help: My freaky protein doesn’t have structure. And it aggregates! A biochemist about to attempt membrane protein purification knows that she is in the beginning of a difficult time in her career. However, there is another wide class of proteins, which are also difficult to purify, because they readily form protein aggregates or are plain insoluble. These are so called ...
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    Dealing with a Stealthy Protein Sample Killer: Shipping on Dry Ice

    This happens all the time: your collaborator ships a protein sample, nicely packed and stored on dry ice, you thaw (more on proper thawing methods in a future blog post) and: the protein is precipitated. There's still plenty of dry ice in the shipping box, sufficient evidence that the sample has not thawed during shipping, and the collaborator insists that ...
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